DETERMINATION OF BIOLOGICALLY ACTIVE COMPOUNDS IN THE “LACTUCA” PREPARATION WITH ANTI-INSOMNIA EFFECTS

Traditional medicine has been recognized as an important field of research in global healthcare, with increasing emphasis on evidence-based validation and standardization. Mongolia has a well-established tradition of utilizing herbal-based formulations as a means of disease prevention and treatment, with insomnia being one of the conditions commonly addressed. This investigation aimed to characterize the phytochemical composition and identify the bioactive compounds in "Lactuca," a recently developed, traditionally inspired herbal preparation. Phytochemical screening was carried out using thin-layer chromatography (TLC) against reference standards. The results showed the presence of polyphenols (tannic acid and caffeic acid), flavonoids (quercetin, kaempferol, and apigenin), alkaloids (piperine), and essential oil (1.8-cineole). The biologically active compounds in Lactuca were determined by spectrophotometry to be polyphenolic compounds at 1.95 mg/mL, total flavonoids at 0.21 mg/mL, and total alkaloids at 2.08 mg/mL. HPLC determined the piperine content to be 0.025 mg/mL.