NEPHROPROTECTIVE EFFECTS OF NEEM GUM (AZADIRACHTA INDICA) ON DIAZINON-INDUCED MALE WISTAR RAT EVALUATED BY BLOOD UREA NITROGEN AND SERUM CREATININE LEVELS

As an agricultural country Indonesia had 7,463,948 hectares of raw rice fields in 2019. The agricultural sector cannot be separated from the use of pesticides. Diazinon, an organophosphate pesticide that is popularly used by farmers has potential harm to human health. Diazinon can cause damage to various organs, one of which is the kidneys. Kidneys play a role in the excretion of diazinon metabolites through urine. Diazinon triggers oxidative stress and triggers kidney damage with changes in biochemical parameters seen from the increase in BUN and serum creatinine levels. Neem (Azadirachta indica) is a plant that can act as an antioxidant. The polysaccharide content in neem gum plays a role in reducing ROS and lipid peroxidation. This study used 30 male wistar rats (Rattus norvegicus) which were divided into 6 groups, namely K (+), K (-), P1, P2, P3 and P4. The study was carried out for 15 days with 7 days of acclimatization and 8 days of treatment. The results showed that the administration of neem gum (Azadirachta indica) at a dose of 15 g/KgBW/day (P3 group) was the group with the most influential dose to prevent kidney damage by reducing BUN levels and neem gum at a dose of 30 g/KgBW/day (P4 group) is the group with the most influential dose to prevent kidney damage by reducing serum creatinine levels in male wistar rats (Rattus norvegicus) induced by diazinon.